Aplysqualenol A binds to the light chain of dynein type 1 (DYNLL1)

Angew Chem Int Ed Engl. 2011 Aug 22;50(35):8134-8. doi: 10.1002/anie.201102546. Epub 2011 Jul 8.

Abstract

A bidirectional affinity system has been developed for the identification of cancer-related natural products and their biological targets. Aplysqualenol A is thus selectively identified as a ligand of the dynein light chain. The use of forward and reverse affinity methods suggests that both small-molecule isolation and target identification can be conducted using conventional molecular biological methods.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Amino Acid Sequence
  • Biological Products / chemistry
  • Biological Products / metabolism
  • Chromatography, Affinity
  • Chromatography, High Pressure Liquid
  • Cytoplasmic Dyneins / chemistry
  • Cytoplasmic Dyneins / metabolism*
  • Furans / chemistry
  • Furans / isolation & purification
  • Furans / metabolism*
  • HCT116 Cells
  • Humans
  • Mass Spectrometry
  • Molecular Sequence Data
  • Protein Binding
  • Pyrans / chemistry
  • Pyrans / isolation & purification
  • Pyrans / metabolism*
  • Surface Plasmon Resonance

Substances

  • Biological Products
  • Furans
  • Pyrans
  • aplysqualenol A
  • DYNLL1 protein, human
  • Cytoplasmic Dyneins