Abstract
A bidirectional affinity system has been developed for the identification of cancer-related natural products and their biological targets. Aplysqualenol A is thus selectively identified as a ligand of the dynein light chain. The use of forward and reverse affinity methods suggests that both small-molecule isolation and target identification can be conducted using conventional molecular biological methods.
Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Amino Acid Sequence
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Biological Products / chemistry
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Biological Products / metabolism
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Chromatography, Affinity
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Chromatography, High Pressure Liquid
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Cytoplasmic Dyneins / chemistry
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Cytoplasmic Dyneins / metabolism*
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Furans / chemistry
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Furans / isolation & purification
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Furans / metabolism*
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HCT116 Cells
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Humans
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Mass Spectrometry
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Molecular Sequence Data
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Protein Binding
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Pyrans / chemistry
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Pyrans / isolation & purification
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Pyrans / metabolism*
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Surface Plasmon Resonance
Substances
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Biological Products
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Furans
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Pyrans
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aplysqualenol A
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DYNLL1 protein, human
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Cytoplasmic Dyneins