A simple, large-scale overexpression method of deriving carbon monoxide dehydrogenase II from thermophilic bacterium Carboxydothermus hydrogenoformans

Biosci Biotechnol Biochem. 2011;75(7):1392-4. doi: 10.1271/bbb.110159. Epub 2011 Jul 7.

Abstract

We established an Na(2)S-free, large-scale overexpression system of deriving CODH II from thermophilic bacterium Carboxydothermus hydrogenoformans in Escherichia coli using a large-scale fermentor. Recombinant-CODH II showed a CO oxidation activity of 9,600 U/mg. In addition, recombinant-CODH II exhibited considerable CO(2) reduction activity, of 16.9 U/mg.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aldehyde Oxidoreductases / isolation & purification*
  • Aldehyde Oxidoreductases / metabolism*
  • Bacteria / chemistry
  • Bacteria / metabolism*
  • Carbon Dioxide / metabolism
  • Carbon Monoxide / metabolism*
  • Fermentation
  • Multienzyme Complexes / isolation & purification*
  • Multienzyme Complexes / metabolism*
  • Oxidation-Reduction
  • Recombinant Proteins / metabolism*

Substances

  • Multienzyme Complexes
  • Recombinant Proteins
  • Carbon Dioxide
  • Carbon Monoxide
  • Aldehyde Oxidoreductases
  • carbon monoxide dehydrogenase