Abstract
We established an Na(2)S-free, large-scale overexpression system of deriving CODH II from thermophilic bacterium Carboxydothermus hydrogenoformans in Escherichia coli using a large-scale fermentor. Recombinant-CODH II showed a CO oxidation activity of 9,600 U/mg. In addition, recombinant-CODH II exhibited considerable CO(2) reduction activity, of 16.9 U/mg.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aldehyde Oxidoreductases / isolation & purification*
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Aldehyde Oxidoreductases / metabolism*
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Bacteria / chemistry
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Bacteria / metabolism*
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Carbon Dioxide / metabolism
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Carbon Monoxide / metabolism*
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Fermentation
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Multienzyme Complexes / isolation & purification*
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Multienzyme Complexes / metabolism*
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Oxidation-Reduction
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Recombinant Proteins / metabolism*
Substances
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Multienzyme Complexes
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Recombinant Proteins
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Carbon Dioxide
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Carbon Monoxide
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Aldehyde Oxidoreductases
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carbon monoxide dehydrogenase