Inhibition of expression of HTLV-1 structural genes mediated by short hairpin RNA in vitro

Anticancer Res. 2011 Jun;31(6):2173-7.

Abstract

Background: Human T-lymphotropic virus I (HTLV-1) is associated with the T-cell malignancy known as adult T-cell leukemia/ lymphoma (ATLL) and with a disorder called HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP). Currently, the treatment of these diseases is based on symptom relief. RNA interference (RNAi) technology has been described as an efficient mechanism for development of new therapeutic methods. Thus, the aim of this study was to evaluate the inhibition of HTLV-1 structural proteins using short hairpin RNAs (shRNAs) expressed by non-viral vectors.

Materials and methods: Reporter plasmids that express enhanced green fluorescent protein-Gag (EGFP-Gag) and EGFP-Env fusion proteins and vectors that express shRNAs corresponding to the HTLV-1 gag and env genes were constructed. shRNA vectors and reporter plasmids were simultaneously transfected into HEK 293 cells.

Results: Fluorescence microscopy, flow cytometry and real-time PCR showed that shRNAs were effective in inhibiting the fusion proteins.

Conclusion: These shRNAs are effective against the expression of structural genes and may provide an approach to the development of new therapeutic agents.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ErbB Receptors / biosynthesis
  • ErbB Receptors / genetics
  • Flow Cytometry
  • Gene Expression Regulation, Viral*
  • Gene Products, env / biosynthesis
  • Gene Products, env / genetics
  • Gene Products, gag / biosynthesis
  • Gene Products, gag / genetics
  • Genetic Vectors / genetics
  • HEK293 Cells
  • Human T-lymphotropic virus 1 / genetics*
  • Humans
  • Microscopy, Fluorescence
  • Polymerase Chain Reaction / methods
  • RNA, Small Interfering / genetics*
  • Recombinant Fusion Proteins / biosynthesis
  • Recombinant Fusion Proteins / genetics
  • Transfection
  • Transformation, Genetic

Substances

  • Gene Products, env
  • Gene Products, gag
  • RNA, Small Interfering
  • Recombinant Fusion Proteins
  • ErbB Receptors