Construction of a 19F-lectin biosensor for glycoprotein imaging by using affinity-guided DMAP chemistry

Bioorg Med Chem Lett. 2011 Aug 1;21(15):4393-6. doi: 10.1016/j.bmcl.2011.06.038. Epub 2011 Jun 17.

Abstract

In this study, assisted by affinity-guided DMAP strategy, we developed a novel (19)F-modified lectin as a biosensor for specific detection and imaging of glycoproteins. Exploited the large chemical shift anisotropy property of (19)F nuclei, glycoproteins detected by our (19)F-biosensor are signatured by broadened peaks in (19)F NMR, hence enabled the distinction between glycoproteins and small molecule saccharides. Such signal on/off switching was also applied to glycoprotein imaging by (19)F MRI.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biosensing Techniques
  • Fluorine Radioisotopes / chemistry
  • Glycoproteins / chemistry
  • Glycoproteins / metabolism*
  • Glycosylation
  • Lectins / chemistry
  • Lectins / metabolism*
  • Magnetic Resonance Spectroscopy
  • Protein Binding
  • Protein Interaction Domains and Motifs
  • Pyridines / chemistry

Substances

  • 4-(dimethylamine)pyridine
  • Fluorine Radioisotopes
  • Glycoproteins
  • Lectins
  • Pyridines