Electrochemical impedance spectroscopy as a highly sensitive tool for a dynamic interaction study between heparin and antithrombin: a novel antithrombin sensor

Abstract

Specific recognition between two biological partners is widely exploited in biosensors nowadays. To explore this avenue, a novel biosensor for antithrombin (AT) detection was constructed. Heparin was used as the affinity ligand. A well-known acrylic monomer (butyl methacrylate) was polymerized and grafted onto the heparin polysaccharide by the use of ceric ammonium nitrate as a redox initiator in aqueous nitric acid medium. Polymers were deposited as a thin layer onto surface of stainless steel electrode (SS316L). The obtained polymers were studied by Fourier transform infrared spectroscopy (FTIR) and analyzed by differential scanning calorimetry (DSC). Moreover, the films were characterized by electrochemical impedance spectroscopy (EIS), contact-angle measurements and AFM. EIS was used to study the biosensor affinity to AT and the relationship between functionalization growth of modified electrode and the response of the sensor. The proposed approach appears to be simple, sensitive and correlated with methods that analyse the detection of antithrombin.