Bile salts induce resistance to polymyxin in enterohemorrhagic Escherichia coli O157:H7

Julianne V Kus; Ahferom Gebremedhin; Vica Dang; Seav-Ly Tran; Anca Serbanescu; Debora Barnett Foster

doi:10.1128/JB.00200-11

Bile salts induce resistance to polymyxin in enterohemorrhagic Escherichia coli O157:H7

J Bacteriol. 2011 Sep;193(17):4509-15. doi: 10.1128/JB.00200-11. Epub 2011 Jul 1.

Authors

Julianne V Kus¹, Ahferom Gebremedhin, Vica Dang, Seav-Ly Tran, Anca Serbanescu, Debora Barnett Foster

Affiliation

¹ Department of Chemistry and Biology, Ryerson University, 350 Victoria St., Toronto, ON M5B 2K3, Canada.

Abstract

Many enteric bacteria use bile as an environmental cue to signal resistance and virulence gene expression. Microarray analysis of enterohemorrhagic Escherichia coli O157:H7 (EHEC) treated with bile salts revealed upregulation of genes for an efflux system (acrAB), a two-component signal transduction system (basRS/pmrAB), and lipid A modification (arnBCADTEF and ugd). Bile salt treatment of EHEC produced a basS- and arnT-dependent resistance to polymyxin.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Outer Membrane Proteins / genetics
Bacterial Outer Membrane Proteins / metabolism
Bile Acids and Salts / pharmacology*
Escherichia coli O157 / genetics*
Escherichia coli O157 / metabolism
Escherichia coli O157 / pathogenicity
Gene Expression Regulation, Bacterial
Genes, Bacterial
Microarray Analysis
Polymyxins / metabolism*
Porins / genetics
Porins / metabolism
Shiga Toxin / genetics
Shiga Toxin / metabolism
Signal Transduction
Up-Regulation

Substances

Bacterial Outer Membrane Proteins
Bile Acids and Salts
Polymyxins
Porins
Shiga Toxin