Antioxidant properties of green tea extract protect reduced fat soft cheese against oxidation induced by light exposure

J Agric Food Chem. 2011 Aug 24;59(16):8718-23. doi: 10.1021/jf201139e. Epub 2011 Jul 29.

Abstract

The effect of two different antioxidants, EDTA and green tea extract (GTE), used individually or in combination, on the light-induced oxidation of reduced fat soft cheeses (0.2 and 6% fat) was investigated. In samples with 0.2% fat, lipid hydroperoxides as primary lipid oxidation products were not detected, but their interference was suggested from the formation of secondary lipid oxidation products such as hexanal and heptanal. The occurrence of these oxidation markers was inhibited by spiking with 50 ppm EDTA or 750 ppm GTE, or a combination of the two prior to irradiation. In contrast, addition of 50 ppm EDTA to samples with 6% fat was ineffective, but 750 ppm GTE (alone or in combination with EDTA) strongly reduced levels of hexanal and heptanal. Accumulation of primary lipid hydroperoxides was not affected by GTE, hence antioxidative activity was ascribed to scavenging of hexanal and heptanal precursors. These radical intermediates result from hydroperoxide disintegration, and subsequent scavenging by GTE, which acts as a radical sink, corroborates the intense signal observed by electron paramagnetic resonance (EPR) spectroscopy.

MeSH terms

  • Aldehydes / analysis
  • Antioxidants / pharmacology*
  • Camellia sinensis / chemistry
  • Cheese / analysis
  • Cheese / radiation effects*
  • Edetic Acid / pharmacology*
  • Light*
  • Lipid Peroxidation / drug effects
  • Oxidation-Reduction
  • Plant Extracts / pharmacology*
  • Tea / chemistry

Substances

  • Aldehydes
  • Antioxidants
  • Plant Extracts
  • Tea
  • heptanal
  • n-hexanal
  • Edetic Acid