Kinetics of catalyzed hydrolysis of 4-methylumbelliferyl caprylate (MUCAP) salmonella reagent

Ahmed S Al-Kady; El-Sadat I Ahmed; M Gaber; Mohamed M Hussein; El-Zeiny M Ebeid

doi:10.1016/j.saa.2011.05.013

Kinetics of catalyzed hydrolysis of 4-methylumbelliferyl caprylate (MUCAP) salmonella reagent

Spectrochim Acta A Mol Biomol Spectrosc. 2011 Sep;79(5):1540-5. doi: 10.1016/j.saa.2011.05.013. Epub 2011 Jun 6.

Authors

Ahmed S Al-Kady¹, El-Sadat I Ahmed, M Gaber, Mohamed M Hussein, El-Zeiny M Ebeid

Affiliation

¹ Chemistry Department, Faculty of Science, Tanta University, Tanta, Egypt.

PMID: 21715222
DOI: 10.1016/j.saa.2011.05.013

Abstract

The kinetics of chemical hydrolysis including neutral, acid- and base-catalyzed hydrolysis of 4-methylumbelliferyl caprylate (MUCAP) salmonella reagent were studied at different temperatures. The rate constants and activation parameters were determined by following the build-up of fluorescence peak of the hydrolysis product 4-methylumbelliferone (4-MU). The time scale of esterase enzyme hydrolysis caused by salmonella was compared with chemical hydrolysis as a background process.

MeSH terms

Bacterial Typing Techniques*
Hydrolysis
Hymecromone / analogs & derivatives*
Hymecromone / chemistry
Kinetics
Salmonella / classification
Salmonella / isolation & purification*
Salmonella Infections / diagnosis*

Substances

4-methylumbelliferyl caprylate
Hymecromone