Involvement of pertussis toxin-sensitive GTP-binding protein in prostaglandin F2 alpha- induced phosphoinositide hydrolysis in osteoblast-like cells

M Miwa; H Tokuda; K Tsushita; J Kotoyori; Y Takahashi; N Ozaki; O Kozawa; Y Oiso

doi:10.1016/0006-291x(90)90817-7

Involvement of pertussis toxin-sensitive GTP-binding protein in prostaglandin F2 alpha- induced phosphoinositide hydrolysis in osteoblast-like cells

Biochem Biophys Res Commun. 1990 Sep 28;171(3):1229-35. doi: 10.1016/0006-291x(90)90817-7.

Authors

M Miwa¹, H Tokuda, K Tsushita, J Kotoyori, Y Takahashi, N Ozaki, O Kozawa, Y Oiso

Affiliation

¹ First Department of Internal Medicine, Nagoya University School of Medicine, Japan.

PMID: 2171509
DOI: 10.1016/0006-291x(90)90817-7

Abstract

Prostaglandin F2 alpha (PGF2 alpha) stimulated the formation of inositol phosphates in a dose-dependent manner in cloned osteoblast-like MC3T3-E1 cells. This reaction was markedly inhibited dose-dependently by pertussis toxin. In the cell membranes, pertussis toxin-catalyzed ADP-ribosylation of a 40-kDa protein was significantly attenuated by pretreatment of PGF2 alpha. These results suggest that pertussis toxin-sensitive GTP-binding protein is involved in the coupling of PGF2 alpha receptor to phospholipase C in these cells.

MeSH terms

Animals
Cell Line
Dinoprost / pharmacology*
GTP-Binding Proteins / metabolism*
Hydrolysis
Inositol Phosphates / metabolism
Kinetics
Membrane Proteins / metabolism
Osteoblasts
Pertussis Toxin*
Phosphatidylinositols / metabolism*
Virulence Factors, Bordetella / pharmacology*

Substances

Inositol Phosphates
Membrane Proteins
Phosphatidylinositols
Virulence Factors, Bordetella
Dinoprost
Pertussis Toxin
GTP-Binding Proteins