Conventional blanket application of nitrogen (N) fertilizer results in more loss of N from soil system and emission of nitrous oxide, a greenhouse gas (GHG). The leaf color chart (LCC) can be used for real-time N management and synchronizing N application with crop demand to reduce GHG emission. A 1-year study was carried out to evaluate the impact of conventional and LCC-based urea application on emission of nitrous oxide, methane, and carbon dioxide in a rice-wheat system of the Indo-Gangetic Plains of India. Treatments consisted of LCC scores of ≤4 and 5 for rice and wheat and were compared with conventional fixed-time N splitting schedule. The LCC-based urea application reduced nitrous oxide emission in rice and wheat. Application of 120 kg N per hectare at LCC ≤ 4 decreased nitrous oxide emission by 16% and methane by 11% over the conventional split application of urea in rice. However, application of N at LCC ≤ 5 increased nitrous oxide emission by 11% over the LCC ≤ 4 treatment in rice. Wheat reduction of nitrous oxide at LCC ≤ 4 was 18% as compared to the conventional method. Application of LCC-based N did not affect carbon dioxide emission from soil in rice and wheat. The global warming potential (GWP) were 12,395 and 13,692 kg CO(2) ha(-1) in LCC ≤ 4 and conventional urea application, respectively. Total carbon fixed in conventional urea application in rice-wheat system was 4.89 Mg C ha(-1) and it increased to 5.54 Mg C ha(-1) in LCC-based urea application (LCC ≤ 4). The study showed that LCC-based urea application can reduce GWP of a rice-wheat system by 10.5%.