In vitro evaluation on the antioxidant capacity of triethylchebulate, an aglycone from Terminalia chebula Retz fruit

Xiuping Chen; Fangyun Sun; Lifeng Ma; Jinhua Wang; Hailin Qin; Guanhua Du

doi:10.4103/0253-7613.81508

In vitro evaluation on the antioxidant capacity of triethylchebulate, an aglycone from Terminalia chebula Retz fruit

Indian J Pharmacol. 2011 May;43(3):320-3. doi: 10.4103/0253-7613.81508.

Authors

Xiuping Chen¹, Fangyun Sun, Lifeng Ma, Jinhua Wang, Hailin Qin, Guanhua Du

Affiliation

¹ Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing.

Abstract

Objectives: To evaluate the antioxidant and free-radical scavenging activities of triethylchebulate (TCL), an aglycone isolated from the fruit of Terminalia chebula Retz.

Materials and methods: Microsomes, mitochondria and red blood cells (RBCs) were isolated from rat liver. The antioxidant capacities were evaluated by determining the inhibitory effects of TCL on lipid peroxidation, hydrogen peroxide (H(2)O(2))-induced RBCs hemolysis and RBCs autoxidative hemolysis. The free-radical scavenging activities were evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) method and 2´,7´-dichlorodihydrofluorescin diacetate (DCFH(2)-DA) assay.

Result: TCL significantly inhibited FeSO(4)/Cys-induced microsomes lipid peroxidation and protected both H(2)O(2-)-induced RBCs hemolysis and RBCs auto-hemolysis in a dose-dependent manner. Furthermore, TCL demonstrated potent DPPH free-radical scavenging ability with IC(50) at 2.4×10(-5) M. In addition, TCL also moderately suppressed azide-induced mitochondria ROS formation.

Conclusion: These results demonstrated that TCL was a strong antioxidant and free-radical scavenger, which might contribute to the anti-oxidative ability of Terminalia chebula Retz.

Keywords: Anti-oxidant; Terminalia chebula Retz; hemolysis; lipid peroxidation; reactive oxygen species; triethylchebulate.