Abstract
The human oral cavity is host to a complex microbial community estimated to comprise >700 bacterial species, of which at least half are thought to be not yet cultivable in vitro. To investigate the plasmids present in this community, we used a transposon-aided capture system, which allowed the isolation of plasmids from human oral supra- and subgingival plaque samples. Thirty-two novel plasmids and a circular molecule that could be an integrase-generated circular intermediate were isolated.
© 2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacteria / classification
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Bacteria / genetics*
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Bacteria / isolation & purification
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Bacterial Typing Techniques
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Base Sequence
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DNA Transposable Elements / genetics
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DNA, Bacterial / analysis*
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DNA, Bacterial / genetics
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DNA, Bacterial / isolation & purification
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Dental Plaque / microbiology*
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Humans
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Molecular Sequence Data
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Periodontal Diseases / genetics
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Periodontal Diseases / microbiology*
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Plasmids / genetics
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Retroelements
Substances
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DNA Transposable Elements
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DNA, Bacterial
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Retroelements
Associated data
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GENBANK/HM560024
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GENBANK/HM560025
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GENBANK/HM560026
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GENBANK/HM560027
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GENBANK/HM560028
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GENBANK/HM560029
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GENBANK/HM560030
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GENBANK/HM560031