Abstract
An important consideration in the selection of a vaccine during the Australian equine influenza (EI) outbreak in 2007 was the ability to differentiate between infected and vaccinated animals (DIVA). A blocking enzyme-linked immunosorbent assay (bELISA) targeted for the nucleoprotein of influenza A viruses was developed to differentiate between naturally infected horses and horses vaccinated with the ProteqFlu® vaccine, which only induces a response to viral haemagglutinin. This bELISA assay met the DIVA requirements and was used extensively during the EI control and eradication programs and 'proof of freedom' testing.
© 2011 The Authors. Australian Veterinary Journal © 2011 Australian Veterinary Association.
MeSH terms
-
Animals
-
Antibodies, Viral / blood
-
Australia
-
Disease Outbreaks / prevention & control
-
Disease Outbreaks / veterinary*
-
Enzyme-Linked Immunosorbent Assay / veterinary
-
Horse Diseases / epidemiology*
-
Horse Diseases / immunology
-
Horse Diseases / prevention & control
-
Horse Diseases / virology*
-
Horses
-
Influenza A Virus, H3N8 Subtype / immunology*
-
Influenza A Virus, H3N8 Subtype / isolation & purification
-
Nucleoproteins / immunology
-
Orthomyxoviridae Infections / epidemiology
-
Orthomyxoviridae Infections / immunology
-
Orthomyxoviridae Infections / prevention & control
-
Orthomyxoviridae Infections / veterinary*
-
Viral Vaccines / administration & dosage
-
Viral Vaccines / immunology
Substances
-
Antibodies, Viral
-
Nucleoproteins
-
Viral Vaccines