Cofermentation of cellobiose and galactose by an engineered Saccharomyces cerevisiae strain

Appl Environ Microbiol. 2011 Aug 15;77(16):5822-5. doi: 10.1128/AEM.05228-11. Epub 2011 Jun 24.

Abstract

We demonstrate improved ethanol yield and productivity through cofermentation of cellobiose and galactose by an engineered Saccharomyces cerevisiae strain expressing genes coding for cellodextrin transporter (cdt-1) and intracellular β-glucosidase (gh1-1) from Neurospora crassa. Simultaneous fermentation of cellobiose and galactose can be applied to producing biofuels from hydrolysates of marine plant biomass.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cellobiose / metabolism*
  • Cellulose / analogs & derivatives
  • Cellulose / metabolism
  • Dextrins / metabolism
  • Ethanol / metabolism
  • Fermentation*
  • Galactose / metabolism*
  • Gene Expression Regulation, Fungal
  • Genetic Engineering*
  • Glucose / metabolism
  • Membrane Transport Proteins / metabolism
  • Neurospora crassa / genetics
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / growth & development
  • Saccharomyces cerevisiae / metabolism*
  • beta-Glucosidase / metabolism

Substances

  • Dextrins
  • Membrane Transport Proteins
  • Cellobiose
  • Ethanol
  • Cellulose
  • cellodextrin
  • beta-Glucosidase
  • Glucose
  • Galactose