High-throughput DNA isolation method for detection of Xylella fastidiosa in plant and insect samples

Jeff A Brady; Jennifer B Faske; Jessica M Castañeda-Gill; Jonathan L King; Forrest L Mitchell

doi:10.1016/j.mimet.2011.06.007

High-throughput DNA isolation method for detection of Xylella fastidiosa in plant and insect samples

J Microbiol Methods. 2011 Sep;86(3):310-2. doi: 10.1016/j.mimet.2011.06.007. Epub 2011 Jun 13.

Authors

Jeff A Brady¹, Jennifer B Faske, Jessica M Castañeda-Gill, Jonathan L King, Forrest L Mitchell

Affiliation

¹ Texas AgriLife Research, Stephenville, TX, USA. j-brady@tamu.edu

PMID: 21703312
DOI: 10.1016/j.mimet.2011.06.007

Abstract

We report an inexpensive, high-throughput method for isolating DNA from insect and plant samples for the purpose of detecting Xylella fastidiosa infection. Existing methods often copurify inhibitors of DNA polymerases, limiting their usefulness for PCR-based detection assays. When compared to commercially available kits, the method provides enhanced pathogen detection at a fraction of the cost.

MeSH terms

Animals
DNA, Bacterial / genetics
DNA, Bacterial / isolation & purification*
High-Throughput Screening Assays / methods*
Insecta / microbiology*
Plant Diseases / microbiology
Plants / microbiology*
Xylella / genetics*
Xylella / isolation & purification*

Substances

DNA, Bacterial