Multiplex real-time PCR assay for detection of methicillin-resistant Staphylococcus aureus and associated toxin genes

J Clin Microbiol. 2011 Aug;49(8):3071-3. doi: 10.1128/JCM.00795-11. Epub 2011 Jun 22.

Abstract

We describe a real-time PCR assay for the detection of methicillin-resistant Staphylococcus aureus and genes encoding toxic shock syndrome toxin 1 and Panton-Valentine leukocidin. Rapid screening and detection of toxins is a useful tool for surveillance studies and outbreak investigations involving large numbers of isolates.

Publication types

  • Evaluation Study

MeSH terms

  • Bacterial Toxins / analysis*
  • Bacterial Toxins / genetics
  • Bacteriological Techniques / methods*
  • DNA, Bacterial / genetics
  • Enterotoxins / analysis*
  • Enterotoxins / genetics
  • Exotoxins / analysis*
  • Exotoxins / genetics
  • Genes, Bacterial
  • Humans
  • Leukocidins / analysis*
  • Leukocidins / genetics
  • Mass Screening / methods
  • Methicillin-Resistant Staphylococcus aureus / genetics
  • Methicillin-Resistant Staphylococcus aureus / isolation & purification*
  • Multiplex Polymerase Chain Reaction / methods*
  • Real-Time Polymerase Chain Reaction / methods*
  • Staphylococcal Infections / microbiology*
  • Superantigens / analysis*
  • Superantigens / genetics

Substances

  • Bacterial Toxins
  • DNA, Bacterial
  • Enterotoxins
  • Exotoxins
  • Leukocidins
  • Panton-Valentine leukocidin
  • Superantigens
  • enterotoxin F, Staphylococcal