Abstract
We report here an ultrasensitive DNA detection approach which combines Au NPs enhanced electrochemiluminescence (ECL) of the CdS nanocrystal (NC) film with isothermal circular amplification reaction of polymerase and nicking endonuclease (NEase). By the double-signal amplification, this approach could sensitively respond down to 5 aM DNA.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cadmium Compounds / chemistry
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DNA / analysis*
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DNA-Directed DNA Polymerase / metabolism
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Electrochemical Techniques
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Endonucleases / metabolism
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Gold / chemistry*
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Luminescent Measurements / methods*
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Metal Nanoparticles / chemistry*
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Sulfides / chemistry
Substances
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Cadmium Compounds
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Sulfides
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cadmium sulfide
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Gold
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DNA
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DNA-Directed DNA Polymerase
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Endonucleases