Objective: The aim of this study was to evaluate, in vitro, the antifungal effect of bioaggregate (BA) against Candida albicans by using the direct contact method.
Study design: BA was tested freshly mixed and after 24-hour set on C. albicans. The tested BA was incubated with C. albicans in plastic tissue clusters for 1 hour, 24 hours, and 3 and 5 days. Aliquots of 0.1 mL were taken from each well at the end of the incubation periods and transfered to tubes containing 5 mL fresh Sabouraud broth. All tubes were vortexed and then incubated at 37°C and observed for the subsequent 5 days. Growth of the fungi was observed daily by the presence of turbidity in the tubes. The results were statistically analyzed by using Kaplan-Meier test.
Results: The freshly mixed and set BA had no antifungal effect at 1 and 24 hours of contact. Both mixes demonstrated complete fungicidal activity after 24 hours' contact. Statistical analysis showed a highly significant difference between the negative and positive control groups (P < .001) and a significant difference between the freshly mixed and 24-hour set BA groups (P < .001) at 24 hours.
Conclusions: BA (freshly mixed and 24-hour set) was effective against C. albicans after 24 hours.
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