Abstract
Elastin-like peptide (ELP) was fused to two different avian flu H5N1 antigens and expressed in transgenic tobacco plants. The presence of the ELP tag enhanced the accumulation of the heterologous proteins in the tobacco leaves. An effective membrane-based Inverse Transition Cycling was developed to recover the ELPylated antigens and antibodies from plant material. The functionality of both the ELPylated neuraminidase and an ELPylated nanobody was demonstrated.
Keywords:
ELPylation; H5N1; Inverse Transition Cycling; avian influenza; nanobody; transgenic plants.
MeSH terms
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Antigens, Viral / genetics
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Antigens, Viral / isolation & purification*
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Antigens, Viral / metabolism
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Elastin / genetics
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Elastin / isolation & purification*
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Elastin / metabolism
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Enzyme-Linked Immunosorbent Assay
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Hemagglutinin Glycoproteins, Influenza Virus / genetics
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Hemagglutinin Glycoproteins, Influenza Virus / isolation & purification
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Hemagglutinin Glycoproteins, Influenza Virus / metabolism
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Influenza A Virus, H5N1 Subtype / genetics*
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Neuraminidase / genetics
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Neuraminidase / isolation & purification
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Neuraminidase / metabolism
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Nicotiana / genetics
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Plant Leaves / genetics
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Plant Leaves / metabolism
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Plants, Genetically Modified / metabolism*
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Recombinant Fusion Proteins
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Transformation, Genetic
Substances
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Antigens, Viral
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Hemagglutinin Glycoproteins, Influenza Virus
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Recombinant Fusion Proteins
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hemagglutinin, avian influenza A virus
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Elastin
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Neuraminidase