PCR and the detection of Pseudomonas aeruginosa in respiratory samples of CF patients. A literature review

Pieter Deschaght; Sabine Van Daele; Frans De Baets; Mario Vaneechoutte

doi:10.1016/j.jcf.2011.05.004

PCR and the detection of Pseudomonas aeruginosa in respiratory samples of CF patients. A literature review

J Cyst Fibros. 2011 Sep;10(5):293-7. doi: 10.1016/j.jcf.2011.05.004.

Authors

Pieter Deschaght¹, Sabine Van Daele, Frans De Baets, Mario Vaneechoutte

Affiliation

¹ Laboratory Bacteriology Research, University of Ghent, De Pintelaan 185, 9000 Ghent, Belgium. Deschaght.Pieter@UGent.be

PMID: 21684819
DOI: 10.1016/j.jcf.2011.05.004

Abstract

Previous studies proved the importance of rapid antibacterial intervention in case of Pseudomonas aeruginosa detection in respiratory samples of cystic fibrosis patients. To improve the early detection of P. aeruginosa, several culture, PCR and serology based approaches have been compared. Because an increasing number of routine microbiology laboratories have access to real-time PCR (qPCR), we reviewed the specificity and sensitivity of published PCR formats. The importance of choice of DNA-extraction methods and PCR formats and of the validation of their specificity and sensitivity with clinical samples is stressed.

Publication types

Review

MeSH terms

Cystic Fibrosis / microbiology*
Humans
Pneumonia, Bacterial / diagnosis
Pneumonia, Bacterial / microbiology
Pseudomonas Infections / diagnosis*
Pseudomonas Infections / microbiology
Pseudomonas aeruginosa / genetics*
Pseudomonas aeruginosa / isolation & purification*
Real-Time Polymerase Chain Reaction*