Abstract
The present study was designed to validate an analytical method based on HPLC for the quantitative measurement of the Apigenin-7-O-β-D-glucoside (AGL) in rat plasma after intravenous administration. HPLC analysis was done using a C18 column, UV detection at 335 nm, and a mobile phase of methanol:0.2% phosphoric acid (1:1, v/v). Good linearity was observed over the range of 0.06-7.20 μg/mL with a lower limit of quantication of 0.06 μg/mL. The intra-and inter-day precision values were below 9.97%. No interference peaks or matrix effects were observed. The method was fully validated and successfully applied to a pharmacokinetic study of AGL.
MeSH terms
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Animals
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Anti-Inflammatory Agents, Non-Steroidal / administration & dosage
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Anti-Inflammatory Agents, Non-Steroidal / blood
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Anti-Inflammatory Agents, Non-Steroidal / isolation & purification
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Anti-Inflammatory Agents, Non-Steroidal / pharmacokinetics
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Antineoplastic Agents, Phytogenic / administration & dosage
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Antineoplastic Agents, Phytogenic / blood
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Antineoplastic Agents, Phytogenic / isolation & purification
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Antineoplastic Agents, Phytogenic / pharmacokinetics
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Antioxidants / administration & dosage
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Antioxidants / analysis*
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Antioxidants / isolation & purification
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Antioxidants / pharmacokinetics*
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Apigenin / administration & dosage
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Apigenin / blood*
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Apigenin / isolation & purification
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Apigenin / pharmacokinetics*
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Calibration
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Chromatography, High Pressure Liquid
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Dose-Response Relationship, Drug
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Drug Stability
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Glucosides / administration & dosage
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Glucosides / blood*
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Glucosides / isolation & purification
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Glucosides / pharmacokinetics*
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Half-Life
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Humulus / chemistry
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Injections, Intravenous
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Limit of Detection
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Metabolic Clearance Rate
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Random Allocation
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Rats
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Rats, Sprague-Dawley
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Reproducibility of Results
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Spectrophotometry, Ultraviolet
Substances
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Anti-Inflammatory Agents, Non-Steroidal
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Antineoplastic Agents, Phytogenic
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Antioxidants
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Glucosides
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apigetrin
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Apigenin