Development of ten polymorphic microsatellite loci for Fosbergia shweliensis (Rubiaceae), a potentially crisis endangered tree

Am J Bot. 2011 Jun;98(6):e161-3. doi: 10.3732/ajb.1100017. Epub 2011 May 27.

Abstract

Premise of the study: Fosbergia shweliensis (Anth.) Tirveng. & Sastre (Rubiaceae) is a potentially endangered tree endemic to China. Microsatellite markers were developed to investigate population genetics of this plant.

Methods and results: Ten polymorphic microsatellite loci were identified and screened in 32 individuals from four wild populations of F. shweliensis. Alleles numbered 2 to 5, and their levels of observed heterozygosity and expected heterozygosity ranged from 0.0564 to 0.7214 and from 0.2794 to 0.7912, respectively.

Conclusions: These new microsatellite loci will facilitate further studies of the population genetics of F. shweliensis, allowing us to design reasonable conservation and management strategies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA Primers / genetics
  • Ecosystem
  • Endangered Species*
  • Flowers / anatomy & histology
  • Genetic Loci / genetics*
  • Genetic Techniques*
  • Microsatellite Repeats / genetics*
  • Molecular Sequence Data
  • Polymorphism, Genetic*
  • Rubiaceae / genetics*
  • Trees / genetics*

Substances

  • DNA Primers

Associated data

  • GENBANK/HQ842847
  • GENBANK/HQ842848
  • GENBANK/HQ842849
  • GENBANK/HQ842850
  • GENBANK/HQ842851
  • GENBANK/HQ842852
  • GENBANK/HQ842853
  • GENBANK/HQ842854
  • GENBANK/HQ842855
  • GENBANK/HQ842856