Templated construction of a Zn-selective protein dimerization motif

Eric N Salgado; Jeffrey D Brodin; Magnus M To; F Akif Tezcan

doi:10.1021/ic200746m

Templated construction of a Zn-selective protein dimerization motif

Inorg Chem. 2011 Jul 4;50(13):6323-9. doi: 10.1021/ic200746m. Epub 2011 Jun 7.

Authors

Eric N Salgado¹, Jeffrey D Brodin, Magnus M To, F Akif Tezcan

Affiliation

¹ Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, MC 0356, La Jolla, California 92093, USA.

Abstract

Here, we report that the approach of metal-templated ligand synthesis can be applied to construct a dimeric protein assembly ((BMOE)RIDC1(2)), which is stabilized by noncovalent interactions and flexible covalent cross-linkers around the Zn templates. Despite its flexibility, (BMOE)RIDC1(2) selectively binds Zn(II) over other divalent metals and undergoes dimerization upon metal binding. Such simultaneous fulfillment of plasticity and selectivity is a hallmark of cellular signaling events that involve ligand/metal-induced protein dimerization.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Cytochromes c / chemistry*
Cytochromes c / metabolism
Ligands
Models, Molecular
Molecular Structure
Organometallic Compounds / chemical synthesis
Organometallic Compounds / chemistry*
Protein Multimerization*
Zinc / chemistry*

Substances

Ligands
Organometallic Compounds
Cytochromes c
Zinc

Grants and funding

T32 GM008326/GM/NIGMS NIH HHS/United States