Detection of bacteria using bacteriophages as recognition elements immobilized on long-period fiber gratings

Opt Express. 2011 Apr 25;19(9):7971-8. doi: 10.1364/OE.19.007971.

Abstract

The paper presents for the first time a study of long-period gratings (LPGs) applied for label-free detection of specific bacteria using physically adsorbed bacteriophages. For the purposes of the experiment a number of highly sensitive LPGs working at the turning point of phase matching curve was fabricated in SMF28 fiber using UV exposure. We show that the device allows for real-time monitoring of phenomena taking place on the sensor's surface, including phage-bacteria interactions. For the applied conditions a resonance wavelength shift of ~1.3 nm induced by bacteria binding was observed.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Adhesion / physiology
  • Bacterial Load / instrumentation*
  • Bacteriophage T4 / physiology*
  • Biological Assay / instrumentation*
  • Biosensing Techniques / instrumentation*
  • Equipment Design
  • Equipment Failure Analysis
  • Escherichia coli / isolation & purification*
  • Escherichia coli / physiology*
  • Refractometry / instrumentation*