Mechanism of lipid extraction from Botryococcus braunii FACHB 357 in a biphasic bioreactor

Fang Zhang; Li-Hua Cheng; Wang-Lei Gao; Xin-Hua Xu; Lin Zhang; Huan-Lin Chen

doi:10.1016/j.jbiotec.2011.05.008

Mechanism of lipid extraction from Botryococcus braunii FACHB 357 in a biphasic bioreactor

J Biotechnol. 2011 Jul 20;154(4):281-4. doi: 10.1016/j.jbiotec.2011.05.008. Epub 2011 May 26.

Authors

Fang Zhang¹, Li-Hua Cheng, Wang-Lei Gao, Xin-Hua Xu, Lin Zhang, Huan-Lin Chen

Affiliation

¹ Department of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou 310027, PR China.

PMID: 21640768
DOI: 10.1016/j.jbiotec.2011.05.008

Abstract

Algal lipid of Botryococcus braunii could be produced continuously and in situ extracted in an aqueous-organic bioreactor. In this study, the cell ultra-structure and cell membrane permeability of B. braunii FACHB 357 were investigated to understand the mechanism of lipid extraction within the biphasic system. The results showed that biocompatible solvent of tetradecane could induce algal lipid accumulation, enable the cell membrane more active and the cell wall much looser. The exocytosis process was observed to be one of the mechanisms for lipid cross-membrane extraction in the presence of organic solvent.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bioreactors*
Chlorophyta / chemistry*
Lipids / isolation & purification*

Substances

Lipids