Abstract
The interaction between fermentation-respiration switch (FrsA) protein and glucose-specific enzyme IIA(Glc) increases glucose fermentation under oxygen-limited conditions. We show that FrsA converts pyruvate to acetaldehyde and carbon dioxide in a cofactor-independent manner and that its pyruvate decarboxylation activity is enhanced by the dephosphorylated form of IIA(Glc) (d-IIA(Glc)). Crystal structures of FrsA and its complex with d-IIA(Glc) revealed residues required for catalysis as well as the structural basis for the activation by d-IIA(Glc).
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetaldehyde / metabolism
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Animals
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Base Sequence
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Carbon Dioxide / metabolism
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Carboxy-Lyases / chemistry
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Carboxy-Lyases / genetics
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Carboxy-Lyases / metabolism*
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Carboxy-Lyases / pharmacology
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Crystallography, X-Ray
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Decarboxylation
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Dose-Response Relationship, Drug
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Electrophoresis, Polyacrylamide Gel
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Escherichia coli / genetics
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Escherichia coli / growth & development
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Escherichia coli / metabolism
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Escherichia coli Proteins / chemistry
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Escherichia coli Proteins / genetics
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Escherichia coli Proteins / metabolism*
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Female
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Fermentation
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Glucose / metabolism*
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Kinetics
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Magnetic Resonance Spectroscopy
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Mice
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Mice, Inbred ICR
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Models, Molecular
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Molecular Sequence Data
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Mutagenesis, Site-Directed
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Phosphoenolpyruvate Sugar Phosphotransferase System / chemistry
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Phosphoenolpyruvate Sugar Phosphotransferase System / genetics
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Phosphoenolpyruvate Sugar Phosphotransferase System / metabolism*
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Protein Conformation
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Pyruvic Acid / metabolism*
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Recombinant Proteins / chemistry
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism*
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Recombinant Proteins / pharmacology
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Substrate Specificity
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Vibrio vulnificus / genetics
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Vibrio vulnificus / metabolism
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Vibrio vulnificus / pathogenicity
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Virulence
Substances
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Escherichia coli Proteins
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Recombinant Proteins
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crr protein, E coli
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Carbon Dioxide
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Pyruvic Acid
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Phosphoenolpyruvate Sugar Phosphotransferase System
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Carboxy-Lyases
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Acetaldehyde
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Glucose
Associated data
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GENBANK/HM172799
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PDB/3MVE
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PDB/3OUR