Rate-limiting steps of tyrosinase-modified electrodes for the detection of catechol

E Burestedt; A Narvaez; T Ruzgas; L Gorton; J Emnéus; E Domínguez; G Marko-Varga

doi:10.1021/ac950742o

Rate-limiting steps of tyrosinase-modified electrodes for the detection of catechol

Anal Chem. 1996 May 1;68(9):1605-11. doi: 10.1021/ac950742o.

Authors

E Burestedt¹, A Narvaez, T Ruzgas, L Gorton, J Emnéus, E Domínguez, G Marko-Varga

Affiliation

¹ Department of Analytical Chemistry, University of Lund, Box 124, S-221 00 Lund, Sweden, Department of Analytical Chemistry, Faculty of Pharmacy, University of Alcalá de Henares, 28 871 Alcalá de Henares, Madrid, Spain, and Enzyme Chemistry Laboratory, Institute of Biochemistry, Mokslininku 12, 2600 Vilnius, Lithuania.

PMID: 21619127
DOI: 10.1021/ac950742o

Abstract

The response currents obtained for tyrosinase-modified Teflon/graphite, carbon paste, and solid graphite electrodes in the presence of catechol are analyzed primarily using rotating disk electrode experiments. The rate-limiting steps, such as the electrochemical reduction of o-quinones and the enzymatic reduction of oxygen as well as the enzymatic oxidation of catechol, are theoretically considered and experimentally demonstrated for the different electrode configurations.