Development of a novel set of microsatellite markers for castor bean, Ricinus communis (Euphorbiaceae)

Am J Bot. 2011 Apr;98(4):e87-9. doi: 10.3732/ajb.1000395. Epub 2011 Mar 30.

Abstract

Premise of study: Microsatellite primers were developed for castor bean (Ricinus communis L.) to investigate genetic diversity and population structure, and to provide support to germplasm management.

Methods and results: Eleven microsatellite loci were isolated using an enrichment cloning protocol and used to characterize castor bean germplasm from the collection at the Instituto Agronômico de Campinas (IAC). In a survey of 76 castor bean accessions, the investigated loci displayed polymorphism ranging from two to five alleles.

Conclusions: The information derived from microsatellite markers led to significant gains in conserved allelic richness and provides support to the implementation of several molecular breeding strategies for castor bean.

MeSH terms

  • Alleles*
  • Cloning, Molecular
  • DNA Primers*
  • DNA, Plant / analysis*
  • Genetic Loci*
  • Microsatellite Repeats*
  • Polymorphism, Genetic*
  • Ricinus / genetics*

Substances

  • DNA Primers
  • DNA, Plant