Abstract
Quercetin's protective effects on the glomerulosclerosis of diabetic nephropathy (DN) in rat mesangial cells were investigated. The cell cycles, type IV collagen and laminin, TGF-β(1) mRNA, Smad 2/3 and Smad 7, and activities of cell antioxidases were measured. Compared with the high glucose group, quercetin may decrease the cell percentages of G(0)/G(1) phase, Smad 2/3 expression, laminin and type IV collagen, and TGF-β(1) mRNA level significantly. The antioxidant capacity, the cell percentages of S phase and Smad 7 expression was significantly increased by quercetin. These results suggest that quercetin is a protective agent against glomerulosclerosis in DN.
Crown Copyright © 2011. Published by Elsevier B.V. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antioxidants / pharmacology*
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Cell Cycle / drug effects
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Cells, Cultured
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Collagen Type IV / drug effects
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Collagen Type IV / metabolism
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Diabetic Nephropathies / metabolism*
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Extracellular Matrix / drug effects*
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Extracellular Matrix / metabolism
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Glucose / adverse effects
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Hypertrophy
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Laminin / drug effects
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Laminin / metabolism
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Mesangial Cells / drug effects*
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Mesangial Cells / metabolism
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Oxidative Stress / drug effects
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Quercetin / pharmacology*
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RNA, Messenger / genetics
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Rats
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Smad2 Protein / drug effects
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Smad2 Protein / metabolism
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Smad3 Protein / drug effects
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Smad3 Protein / metabolism
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Smad7 Protein / drug effects
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Smad7 Protein / metabolism
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Transforming Growth Factor beta1 / drug effects
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Transforming Growth Factor beta1 / genetics
Substances
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Antioxidants
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Collagen Type IV
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Laminin
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RNA, Messenger
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Smad2 Protein
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Smad2 protein, rat
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Smad3 Protein
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Smad3 protein, rat
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Smad7 Protein
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Smad7 protein, rat
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Transforming Growth Factor beta1
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Quercetin
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Glucose