Comparison of metabolite profiles in U87 glioma cells and mesenchymal stem cells

Kathrin Jürchott; Ke-Tai Guo; Gareth Catchpole; Kristen Feher; Lothar Willmitzer; Christian Schichor; Joachim Selbig

doi:10.1016/j.biosystems.2011.05.005

Comparison of metabolite profiles in U87 glioma cells and mesenchymal stem cells

Biosystems. 2011 Aug;105(2):130-9. doi: 10.1016/j.biosystems.2011.05.005. Epub 2011 May 14.

Authors

Kathrin Jürchott¹, Ke-Tai Guo, Gareth Catchpole, Kristen Feher, Lothar Willmitzer, Christian Schichor, Joachim Selbig

Affiliation

¹ AG Bioinformatics, Institute for Biochemistry and Biology, University of Potsdam, Germany. juerchott@mpimp-golm.mpg.de

PMID: 21605622
DOI: 10.1016/j.biosystems.2011.05.005

Abstract

Gas chromatography-mass spectrometry (GC-MS) profiles were generated from U87 glioma cells and human mesenchymal stem cells (hMSC). 37 metabolites representing glycolysis intermediates, TCA cycle metabolites, amino acids and lipids were selected for a detailed analysis. The concentrations of these metabolites were compared and Pearson correlation coefficients were used to calculate the relationship between pairs of metabolites. Metabolite profiles and correlation patterns differ significantly between the two cell lines. These profiles can be considered as a signature of the underlying biochemical system and provide snap-shots of the metabolism in mesenchymal stem cells and tumor cells.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Amino Acids / metabolism*
Cell Line
Computational Biology
Gas Chromatography-Mass Spectrometry / methods
Glioma / metabolism*
Humans
Lipid Metabolism*
Mesenchymal Stem Cells / metabolism*
Metabolic Networks and Pathways*

Substances

Amino Acids