Multiple ST clonal complexes, with a predominance of ST131, of Escherichia coli harbouring blaCTX-M-15 in a tertiary hospital in Tanzania

Clin Microbiol Infect. 2011 Aug;17(8):1279-82. doi: 10.1111/j.1469-0691.2011.03518.x. Epub 2011 May 20.

Abstract

The molecular epidemiology of 32 non-duplicate, CTX-M-15 extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli strains, isolated from clinical samples, was investigated. Multilocus sequence typing revealed multiple sequence type clonal complexes: ST131 (12), ST405 (4), ST638 (3), ST38 (2), ST827 (2), ST224 (1), ST648 (1), ST46 (1) and two new sequence type clonal complexes (1845 and 1848) in 22 pulsed field gel electrophoresis clusters. The bla(CTX-M-15) gene was located on conjugative IncF plasmids. This is the first report of the worldwide emerging clonal complex ST131 linked to bla(CTX-M-15) in Tanzania and demonstrates the need for constant surveillance in developing countries to prevent the spread of these multiresistant isolates.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / pharmacology
  • Bacterial Typing Techniques
  • Electrophoresis, Gel, Pulsed-Field
  • Escherichia coli / drug effects
  • Escherichia coli / genetics*
  • Escherichia coli / isolation & purification
  • Escherichia coli Infections / epidemiology*
  • Escherichia coli Infections / microbiology
  • Hospitals / statistics & numerical data*
  • Humans
  • Microbial Sensitivity Tests
  • Molecular Epidemiology*
  • Multilocus Sequence Typing
  • Plasmids / genetics*
  • Polymerase Chain Reaction
  • Tanzania / epidemiology
  • beta-Lactamases / genetics*

Substances

  • Anti-Bacterial Agents
  • beta-lactamase CTX-M-15
  • beta-Lactamases