This section of Cystic Fibrosis: Diagnosis and Protocols is focussed on methods aimed at detecting expression, localization, endocytic sorting and metabolism (biogenesis and turnover), as well as interacting partners of the cystic fibrosis transmembrane conductance regulator (CFTR), the protein product of the gene mutated in cystic fibrosis (CF). An overview of the protocols to be found in subsequent chapters of this book section is provided here, as well as the rationale for utilizing these protocols (also as a workflow) explaining which scientific question(s) each of them helps to address. Protocols included in other sections of this book are also cross-referenced.