Abstract
Lighter cells from density fractionated erythrocytes of sickle cell disease (SCD) patients carry higher amount of externalized phosphatidylserine (PS) and cell surface glycophorins compared to the denser counterparts. Further analysis also revealed that the denser cells contained higher levels of fetal hemoglobin (HbF) compared to the lighter cells, supported by the presence of larger number of F-cells in these populations. In this report, we have found direct evidence on the higher survival of the HbF rich erythrocytes in SCD.
Copyright © 2011. Published by Elsevier Inc.
MeSH terms
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Adult
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Anemia, Sickle Cell / metabolism*
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Anemia, Sickle Cell / pathology
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Annexin A5 / analysis
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Cell Count
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Cell Membrane / chemistry*
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Cell Membrane / metabolism
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Cell Separation
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Child
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Erythrocyte Aging
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Erythrocyte Count
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Erythrocytes / metabolism*
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Erythrocytes / pathology
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Fetal Hemoglobin / analysis*
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Flow Cytometry
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Glycophorins / analysis
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Humans
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Phosphatidylserines / analysis
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Povidone
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Sickle Cell Trait / metabolism*
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Sickle Cell Trait / pathology
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Silicon Dioxide
Substances
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Annexin A5
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Glycophorins
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Phosphatidylserines
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Percoll
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Silicon Dioxide
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Fetal Hemoglobin
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Povidone