In the title solvate, C(30)H(39)NO(5)·CH(4)O {systematic name: 21-acet-oxy-18,21-dihy-droxy-5,6,16,18-tetra-methyl-10-phenyl-6,7-ep-oxy-[11]cytochalasa-13,19-dien-1-one methanol solvate}, the organic mol-ecule exhibits the tetra-cyclic terpenoid skeleton of cytochalasin, consisting of fused five-, six-, three- and 11-membered rings. The five-membered ring adopts an envelope conformation, while the six-membered ring is in a boat conformation. The ep-oxy O atom on the six-membered ring is pointing away from the five-membered ring. An inter-stitial methanol solvent mol-ecule is hydrogen bonded to the cytochalasin mol-ecules and inter-molecular O-H⋯O and N-H⋯O hydrogen bonds connect the mol-ecules into infinite chains along the (10) direction.