The biosecurity of transgenic organism has been widely concerned and extremely restricted its application. Recently, many technological strategies have been developed to ensure its biosecurity. Thus, transgene containment technologies have become one of the hotspots in current transgenic research. In this paper, several transgene containment technologies, such as marker-free transgenic technology, safety marker transgenic technology, chloroplast transgenic technologies, terminator technology, male sterility technology, and 'GM-gene-deletor'technology were reviewed and evaluated. 'GM-gene-deletor' technology, as one of these technologies, demonstrated a prosperous future for safe application of transgenic organisms. Finally, the strategies for developing new transgene containment technologies have been suggested.