Isolation and characterization of microsatellite markers in the newly discovered invasive fruit fly pest in Africa, Bactrocera invadens (Diptera: Tephritidae)

F Khamis; N Karam; C R Guglielmino; S Ekesi; D Masiga; M DE Meyer; E U Kenya; A R Malacrida

doi:10.1111/j.1755-0998.2008.02335.x

Isolation and characterization of microsatellite markers in the newly discovered invasive fruit fly pest in Africa, Bactrocera invadens (Diptera: Tephritidae)

Mol Ecol Resour. 2008 Nov;8(6):1509-11. doi: 10.1111/j.1755-0998.2008.02335.x.

Authors

F Khamis¹, N Karam, C R Guglielmino, S Ekesi, D Masiga, M DE Meyer, E U Kenya, A R Malacrida

Affiliation

¹ International Centre of Insect Physiology and Ecology, PO Box 30772-00100 GPO, Nairobi, Kenya, Kenyatta University, PO Box 43844-00100 GPO, Nairobi, Kenya, Dipartimento di Biologia Animale, Università di Pavia, Piazza Botta 9, 27100 Pavia, Italy, Dipartimento di Genetica e Microbiologia, Università di Pavia, via Ferrata 1, 27100 Pavia, Italy, Entomology Section, Royal Museum for Central Africa, Leuvensesteenweg 13, B-3080 Tervuren, Belgium.

PMID: 21586091
DOI: 10.1111/j.1755-0998.2008.02335.x

Abstract

We describe the isolation and characterization of 11 polymorphic microsatellite loci from the recently discovered fruit fly pest, Bactrocera invadens. The polymorphism of these loci was tested in individual flies from two natural populations (Sri Lanka and Democratic Republic of Congo). Allele number per locus ranged from three to 15 and eight loci displayed a polymorphic information content greater than 0.5. These microsatellite loci provide useful markers for studies of population dynamics and invasion history of this pest species.