Cross-species amplification of Eucalyptus microsatellite loci

Paul G Nevill; Alastair Reed; Gerd Bossinger; Rene E Vaillancourt; Mathew Larcombe; Peter K Ades

doi:10.1111/j.1755-0998.2008.02362.x

Cross-species amplification of Eucalyptus microsatellite loci

Mol Ecol Resour. 2008 Nov;8(6):1277-80. doi: 10.1111/j.1755-0998.2008.02362.x. Epub 2008 Sep 23.

Authors

Paul G Nevill¹, Alastair Reed, Gerd Bossinger, Rene E Vaillancourt, Mathew Larcombe, Peter K Ades

Affiliation

¹ School of Forest and Ecosystem Science and Cooperative Research Centre for Forestry, University of Melbourne, Water Street, Creswick, Victoria 3363, Australia, School of Plant Science and Cooperative Research Centre for Forestry, University of Tasmania, Private Bag 55, Hobart, Tasmania 7001, Australia, School of Forest and Ecosystem Science and Cooperative Research Centre for Forestry, University of Melbourne, Parkville, Victoria 3010, Australia.

PMID: 21586020
DOI: 10.1111/j.1755-0998.2008.02362.x

Abstract

This study examined the interspecific amplification of nuclear microsatellite loci developed mainly for eucalypts in the subgenus Symphyomyrtus across five species within the second most speciose subgenus, subgenus Eucalyptus. A set of eight to 10 loci, depending on taxon, have been identified that are highly variable and easily scored. The successful transfer of microsatellite loci to these eucalypt species sidesteps the expensive and time-consuming development of species-specific microsatellite libraries. This primer set will enable the examination and cross-species comparison of the genetic resources of commercially and ecologically important members of the subgenus Eucalyptus.