PERMANENT GENETIC RESOURCES: Isolation and characterization of microsatellite DNA loci from the southern flounder, Paralichthys lethostigma

C W Shao; X Liao; S L Chen

doi:10.1111/j.1471-8286.2007.01965.x

PERMANENT GENETIC RESOURCES: Isolation and characterization of microsatellite DNA loci from the southern flounder, Paralichthys lethostigma

Mol Ecol Resour. 2008 Mar;8(2):381-3. doi: 10.1111/j.1471-8286.2007.01965.x.

Authors

C W Shao¹, X Liao, S L Chen

Affiliation

¹ Key Laboratory for Sustainable Utilization of Marine Fisheries Resource, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China, Division of Life Science and Technology, Ocean University of China, Qingdao 266003, China.

PMID: 21585798
DOI: 10.1111/j.1471-8286.2007.01965.x

Abstract

Paucity of polymorphic molecular markers in southern flounder (Paralichthys lethostigma) has been a major limitation in genetic improvement of this important economic fish. Hence, we constructed a repeat-enriched genomic library from P. lethostigma. A total of 39 new microsatellites were identified, for which 33 primer pairs were designed. After validating and scoring, 10 of these loci were polymorphic in a test population with the range of alleles from two to nine per locus. The observed and expected heterozygosities ranged from 0.2500 to 0.9000 and from 0.4469 to 0.8514, respectively. These polymorphic microsatellites will be useful for genetic diversity analysis and linkage map construction for P. lethostigma.