PERMANENT GENETIC RESOURCES: Isolation of microsatellite loci from the endangered plant Sibara filifolia (Brassicaceae)

Mitchell E McGlaughlin; Lisa E Wallace; Kaius Helenurm

doi:10.1111/j.1471-8286.2007.01958.x

PERMANENT GENETIC RESOURCES: Isolation of microsatellite loci from the endangered plant Sibara filifolia (Brassicaceae)

Mol Ecol Resour. 2008 Mar;8(2):367-9. doi: 10.1111/j.1471-8286.2007.01958.x.

Authors

Mitchell E McGlaughlin¹, Lisa E Wallace, Kaius Helenurm

Affiliation

¹ Department of Biology, University of South Dakota, CL 191, 414 East Clark Street, Vermillion, SD 57069, USA Department of Biological Sciences, Mississippi State University, Mississippi State, MS 39762, USA.

PMID: 21585794
DOI: 10.1111/j.1471-8286.2007.01958.x

Abstract

Sibara filifolia (Brassicaceae) is a federally endangered annual herb found on two of the California Channel Islands. Previous studies based on allozymes revealed little genetic variability on San Clemente Island. Nine polymorphic microsatellite loci were isolated from individuals on San Clemente Island. We found low levels of allelic variation (mean N(A) = 2.3), with seven loci exhibiting significant deviations from Hardy-Weinberg equilibrium (P < 0.01) and 10 pairs of loci exhibiting significant linkage disequilibrium (P < 0.01). Most of the observed variability (mean H(O) = 0.003) occurred among populations or in rare homozygous individuals.