PERMANENT GENETIC RESOURCES: Development of microsatellite markers of the Mexican understorey palm Chamaedorea elegans, cross-species genotyping, and amplification in congeners

Angélica Cibrián-Jaramillo; William J Hahn; Rob Desalle

doi:10.1111/j.1471-8286.2007.01942.x

PERMANENT GENETIC RESOURCES: Development of microsatellite markers of the Mexican understorey palm Chamaedorea elegans, cross-species genotyping, and amplification in congeners

Mol Ecol Resour. 2008 Mar;8(2):322-4. doi: 10.1111/j.1471-8286.2007.01942.x.

Authors

Angélica Cibrián-Jaramillo¹, William J Hahn, Rob Desalle

Affiliation

¹ Department of Ecology, Evolution, and Environmental Biology, Columbia University, 1200 Amsterdam Avenue, MC5557, New York, NY 10027, USA, Department of Biology, Georgetown University, 37th & O Streets, NW, Washington, D.C. 20057, USA, Division of Invertebrate Zoology and Sackler Institute for Comparative Genomics, American Museum of Natural History, Central Park West at 79th Street, New York, NY 10024, USA.

PMID: 21585780
DOI: 10.1111/j.1471-8286.2007.01942.x

Abstract

With striking morphological diversity and adaptability, Chamaedorea palms constitute an ecologically and economically important understorey component of Neotropical forests. Nine loci developed for Chamaedorea elegans evaluated in three Veracruz populations resulted in a large number of alleles (8-18), and high expected heterozygosity (0.49-0.92), but low observed (0.27-0.65) heterozygosity. Deviations from Hardy-Weinberg and high inbreeding suggest a lack of panmixia. Eight loci optimized for Chamaedorea ernesti-augustii showed similar patterns of variation. All nine multiplexing loci amplified in other five congeneric species, which will facilitate comparisons within the genus and contribute to the conservation of its genetic resources.