Abstract
In the present study, we investigated the function of the heterotrimeric G protein β-subunit (Gβ) gene (RGB1) in rice. RGB1 knock-down lines were generated in the wild type and d1-5, a mutant deficient for the heterotrimeric G protein α-subunit (Gα) gene (RGA1). Both transgenic lines showed browning of the lamina joint regions and nodes that could be attributed to a reduction of RGB1 function, as the abnormality was not observed in d1-5. The RGB1 knock-down lines generated in d1-5 were shorter, suggesting RGB1 to be a positive regulator of cellular proliferation, in addition to RGA1. The number of sterile seeds also increased in both RGB1 knock-down lines. These results suggest that Gβγ and Gα cooperatively function in cellular proliferation and seed fertility. We discuss the potential predominant role of RGB1 in G protein signaling in rice.
© 2011 The Authors. The Plant Journal © 2011 Blackwell Publishing Ltd.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cell Proliferation
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DNA, Plant / genetics
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Flowers / anatomy & histology
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Flowers / genetics
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Flowers / physiology
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GTP-Binding Protein alpha Subunits / genetics
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GTP-Binding Protein alpha Subunits / metabolism*
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GTP-Binding Protein beta Subunits / genetics
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GTP-Binding Protein beta Subunits / metabolism*
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Gene Expression Regulation, Plant / physiology
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Oryza / anatomy & histology
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Oryza / genetics
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Oryza / physiology*
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Phenotype
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Plant Leaves / genetics
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Plant Leaves / growth & development
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Plant Leaves / physiology
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Plant Proteins / genetics
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Plant Proteins / metabolism
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Plant Roots / anatomy & histology
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Plant Roots / genetics
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Plant Roots / physiology
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Plant Stems / anatomy & histology
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Plant Stems / genetics
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Plant Stems / physiology
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Plants, Genetically Modified / anatomy & histology
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Plants, Genetically Modified / genetics
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Plants, Genetically Modified / physiology
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Promoter Regions, Genetic / genetics
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RNA Interference
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RNA, Messenger / genetics
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RNA, Plant / genetics
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Seedlings / anatomy & histology
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Seedlings / genetics
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Seedlings / physiology
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Seeds / anatomy & histology
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Seeds / genetics
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Seeds / physiology*
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Sequence Deletion
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Signal Transduction / physiology
Substances
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DNA, Plant
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GTP-Binding Protein alpha Subunits
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GTP-Binding Protein beta Subunits
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Plant Proteins
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RNA, Messenger
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RNA, Plant