Abstract
The structures of the ribosomally synthesized peptide antibiotics from Bacillus amyloliquefaciens FZB42, plantazolicin A and B, have been elucidated by high resolving ESI-MSMS, 2D (1)H-(13)C-correlated NMR spectroscopy as well as (1)H-(15)N-HMQC/(1)H-(15)N-HMBC NMR experiments. (15)N-labeling prior to the experiments facilitated the structure determination, unveiling a hitherto unusual number of thiazoles and oxazoles formed from a linear 14mer precursor peptide. This finding further extends the number of known secondary metabolites from B. amyloliquefaciens and represents a new type of secondary metabolites from the genus Bacillus.
© 2011 American Chemical Society
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anti-Bacterial Agents / chemistry
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Anti-Bacterial Agents / isolation & purification*
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Anti-Bacterial Agents / pharmacology
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Bacillus / chemistry*
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Bacillus / genetics
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Microbial Sensitivity Tests
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Molecular Structure
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Nuclear Magnetic Resonance, Biomolecular
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Oligopeptides / chemistry
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Oligopeptides / isolation & purification*
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Oligopeptides / metabolism
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Oxazoles / chemistry
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Oxazoles / isolation & purification*
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Oxazoles / pharmacology
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Ribosomes / metabolism
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Thiazoles / chemistry
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Thiazoles / isolation & purification*
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Thiazoles / pharmacology
Substances
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Anti-Bacterial Agents
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Oligopeptides
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Oxazoles
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Thiazoles
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plantazolicin A
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plantazolicin B