Coupling non-destructive DNA extraction and voucher retrieval for small soft-bodied Arthropods in a high-throughput context: the example of Collembola

David Porco; Rodolphe Rougerie; Louis Deharveng; Paul Hebert

doi:10.1111/j.1755-0998.2010.2839.x

Coupling non-destructive DNA extraction and voucher retrieval for small soft-bodied Arthropods in a high-throughput context: the example of Collembola

Mol Ecol Resour. 2010 Nov;10(6):942-5. doi: 10.1111/j.1755-0998.2010.2839.x.

Authors

David Porco¹, Rodolphe Rougerie, Louis Deharveng, Paul Hebert

Affiliation

¹ Canadian Center of DNA Barcoding, University of Guelph, 50 Stone Road East, N1G 2W1, Guelph, Ontario, Canada UMR 5202 CNRS "Origine, Structure et Evolution de la Biodiversité", Muséum National d'Histoire Naturelle, C.P.50 45 rue Buffon, 75005 Paris, France.

PMID: 21565103
DOI: 10.1111/j.1755-0998.2010.2839.x

Abstract

Here, we describe a simple method adapted for high-throughput protocols allowing voucher specimen recovery for Collembola and by extension for other soft-bodied small arthropods. A standard extraction protocol was tested to examine the effects of lysis duration (1, 2, 4, 12 h) on DNA concentration, amplification success and specimen condition. Good quality DNA was obtained after 1 h of lysis, while voucher condition was fine for up to 12 h. The lysis step substantially shortens the clearing process necessary for morphological examination.