Identification of single copy nuclear DNA markers for North American pit vipers

H Lisle Gibbs; Jose Diaz

doi:10.1111/j.1755-0998.2009.02707.x

Identification of single copy nuclear DNA markers for North American pit vipers

Mol Ecol Resour. 2010 Jan;10(1):177-80. doi: 10.1111/j.1755-0998.2009.02707.x. Epub 2009 Apr 23.

Authors

H Lisle Gibbs¹, Jose Diaz

Affiliation

¹ Department of Evolution, Ecology and Organismal Biology, Ohio State University, Columbus, OH 43210-1293, USA.

PMID: 21565006
DOI: 10.1111/j.1755-0998.2009.02707.x

Abstract

We describe 18 single copy nuclear DNA loci (10 loci cloned from a Sistrurus catenatus catenatus genomic library and eight intron-based loci amplified using conserved primers) that detect sequence variation in species from all genera (Sistrurus, Agkistrodon and Crotalus) of North American pit viper snakes. These loci (mean size in bp ± SE: 433 ± 51) show large numbers of phylogenetically informative sites across species (mean + SE: 10.2 ± 1.5), but limited variation within subspecies suggesting that they will be most useful for multilocus species-level phylogenetic analyses in these snakes.