Isolation and evaluation of 18 microsatellite markers in Siniperca chuatsi (Basilewsky)

Gangqiao Kuang; Shuangqing Lu; Shuming Zheng; Qing Wu

doi:10.1111/j.1755-0998.2009.02700.x

Isolation and evaluation of 18 microsatellite markers in Siniperca chuatsi (Basilewsky)

Mol Ecol Resour. 2009 Nov;9(6):1473-5. doi: 10.1111/j.1755-0998.2009.02700.x. Epub 2009 Apr 23.

Authors

Gangqiao Kuang¹, Shuangqing Lu, Shuming Zheng, Qing Wu

Affiliation

¹ Department of Fisheries, Rongchang Campus, Southwest University, Rongchang, Chongqing 402460, China Department of Biotechnology and Environment Science, Changsha University, Changsha 410003, China.

PMID: 21564936
DOI: 10.1111/j.1755-0998.2009.02700.x

Abstract

Eighteen novel microsatellite markers were isolated in the Chinese perch Siniperca chuatsi (Basilewsky) using the FIASCO method, from (AC/TG)(n) , (AG/TC)(n) , (AT/TA)(n) , (GATA/CTAT)(n) and (GATT/CTAA)(n) repeat genomic libraries. The number of alleles per locus ranged from two to eight in a sample of 30 individuals from a wild population. Observed heterozygosity was between 0.100 and 0.737. Seven loci showed significant deviation from Hardy-Weinberg equilibrium, null alleles were suggested at nine loci but no linkage disequilibrium between loci was detected. These loci could be useful in the population genetic study of S. chuatsi.