Isolation and characterization of microsatellite markers from Teretrius nigrescens Lewis (Coleoptera: Histeridae), predator of the storage pest Prostephanus truncatus (Horn) (Coleoptera: Bostrichidae)

A B Omondi; L C Orantes; J VAN DEN Berg; D Masiga; F Schulthess

doi:10.1111/j.1755-0998.2009.02613.x

Isolation and characterization of microsatellite markers from Teretrius nigrescens Lewis (Coleoptera: Histeridae), predator of the storage pest Prostephanus truncatus (Horn) (Coleoptera: Bostrichidae)

Mol Ecol Resour. 2009 Jul;9(4):1236-9. doi: 10.1111/j.1755-0998.2009.02613.x. Epub 2009 Mar 5.

Authors

A B Omondi¹, L C Orantes, J VAN DEN Berg, D Masiga, F Schulthess

Affiliation

¹ International Centre of Insect Physiology and Ecology, PO Box 30772, Nairobi, Kenya.

PMID: 21564888
DOI: 10.1111/j.1755-0998.2009.02613.x

Abstract

Teretrius nigrescens is a predator of the larger grain borer (LGB) Prostephanus truncatus, an invasive post-harvest pest in Africa. We describe the isolation and characterization of 24 novel polymorphic microsatellite markers and their testing on a population from Honduras. Alleles per locus ranged between 2 and 12, and observed heterozygosity between 0.037 and 0.646. Six loci deviated significantly from Hardy-Weinberg equilibrium and showed evidence of null alleles. These markers will be useful for studies of the predator's population structure and characterizing populations for control of LGB.