Isolation of nine nuclear microsatellites in the common Mediterranean sea urchin, Paracentrotus lividus (Lamarck)

I Calderón; X Turon; M Pascual

doi:10.1111/j.1755-0998.2009.02585.x

Isolation of nine nuclear microsatellites in the common Mediterranean sea urchin, Paracentrotus lividus (Lamarck)

Mol Ecol Resour. 2009 Jul;9(4):1145-7. doi: 10.1111/j.1755-0998.2009.02585.x. Epub 2009 Feb 24.

Authors

I Calderón¹, X Turon, M Pascual

Affiliation

¹ Department of Animal Biology (Invertebrates), Faculty of Biology, University of Barcelona, 645 Diagonal Avenue, 08028 Barcelona, Spain. calderon@ub.edu

PMID: 21564856
DOI: 10.1111/j.1755-0998.2009.02585.x

Abstract

Nine polymorphic microsatellite loci were isolated and characterized for the edible common sea urchin Paracentrotus lividus. Loci were obtained from two genomic libraries enriched with different di-, tri- and tetranucleotides. Most microsatellites obtained were imperfect dinucleotides. Allelic variation was screened for a total of 56 individuals from two populations from North-Western Mediterranean. Microsatellites showed a number of alleles ranging from 13 to 35. Linkage disequilibrium was not detected between any pair of loci, and all loci showed a significant departure from Hardy-Weinberg equilibrium which is not likely to be the result of null alleles, suggesting that demographic features may be acting upon this commercially interesting species.