Microsatellite loci within sixgill sharks, Hexanchus griseus

Abstract

Novel sixgill shark (Hexanchus griseus) microsatellite loci were developed and tested on five shark species. A suite of microsatellite loci previously developed for lemon sharks (Negaprion brevirostris) was also tested. Data on 15 microsatellites are presented including primer sequences, number of alleles (a), observed (H(O) ) and expected heterozygosities (H(E) ), and F(IS) values for sixgill sharks (a = 10-69, H(O) = 0.24-1.00, H(E) = 0.76-0.96 and F(IS) = -0.21-0.60), sevengill sharks (Notorynchus cepedianus) (a = 6-40, H(O) = 0.20-0.73, H(E) = 0.59-0.94 and F(IS) = -0.47-0.58), Pacific spiny dogfish (Squalus acanthias) (a = 3-13, H(O) = 0.00-0.96, H(E) = 0.24-0.93 and F(IS) =-0.52-1.00), angle sharks (Squatina californica) (a = 1-4, H(O) = 0.00-1.00, H(E) = 0.60-1.00 and F(IS) =-1.00-0.25), and leopard sharks (Triakis semifasciata) (a = 3-16, H(O) = 0.20-1.00, H(E) = 0.53-0.92 and F(IS) = -0.57-1.00). A final suite of 14 microsatellites (13 developed from sixgill sharks and one from lemon sharks) were found to be polymorphic and conform to Hardy-Weinberg equilibrium within sixgill sharks.