Comprehensive cross-amplification of microsatellite multiplex sets across the rodent genus Microtus

M Rudá; D Ziak; B Gauffre; J Zima; N Martínková

doi:10.1111/j.1755-0998.2009.02521.x

Comprehensive cross-amplification of microsatellite multiplex sets across the rodent genus Microtus

Mol Ecol Resour. 2009 May;9(3):974-8. doi: 10.1111/j.1755-0998.2009.02521.x. Epub 2009 Feb 3.

Authors

M Rudá¹, D Ziak, B Gauffre, J Zima, N Martínková

Affiliation

¹ Institute of Vertebrate Biology, Academy of Science of the Czech Republic, v.v.i., Květná 8, 603 65 Brno, Czech Republic Department of Zoology, Faculty of Natural Sciences, Comenius University, Mlynská dolina B2, 842 15 Bratislava, Slovakia CBGP-INRA, Campus International de Baillarguet, CS 30016, 34988 Montferrier-sur-Lez Cedex, France.

PMID: 21564810
DOI: 10.1111/j.1755-0998.2009.02521.x

Abstract

We developed four multiplex panels comprising 19 microsatellite loci and tested their amplification in 21 rodent species important for agricultural and conservation management (Microtus, Arvicola, Chionomys). On average, 17.6 loci amplified per species. Number of alleles ranged from 1 to 19 per locus. We report an additional locus polymorphic in 15 vole species.