Characterization of 24 microsatellite loci in delta smelt, Hypomesus transpacificus, and their cross-species amplification in two other smelt species of the Osmeridae family

Kathleen M Fisch; Jessica L Petersen; Melinda R Baerwald; John K Pedroia; Bernie May

doi:10.1111/j.1755-0998.2008.02254.x

Characterization of 24 microsatellite loci in delta smelt, Hypomesus transpacificus, and their cross-species amplification in two other smelt species of the Osmeridae family

Mol Ecol Resour. 2009 Jan;9(1):405-8. doi: 10.1111/j.1755-0998.2008.02254.x.

Authors

Kathleen M Fisch¹, Jessica L Petersen, Melinda R Baerwald, John K Pedroia, Bernie May

Affiliation

¹ Department of Animal Science, University of California - Davis, 1 Shields Avenue, Davis, CA 95616, USA.

PMID: 21564663
DOI: 10.1111/j.1755-0998.2008.02254.x

Abstract

We characterized 24 polymorphic tetranucleotide microsatellite loci for delta smelt (Hypomesus transpacificus) endemic to the San Francisco Bay Estuary, CA, USA. Screening of samples (n = 30) yielded two to 26 alleles per locus with observed levels of heterozygosity ranging from 0.17 to 1.0. Only one locus deviated from Hardy-Weinberg equilibrium, suggesting these individuals originate from a single panmictic population. Linkage disequilibrium was found in two pairs of loci after excluding the locus out of Hardy-Weinberg equilibrium. Twenty-two primer pairs cross-amplified in wakasagi smelt (Hypomesus nipponensis), and 15 primer pairs cross-amplified in longfin smelt (Spirinchus thaleichthys).